Key to the list of RAPD loci and abbreviations used
Various columns:
- Slashes ("/") separate information for two RAPDs, which are
determined by the same PCR primer combination and which behave as
allelic to one another; thus, they behave like a codominant RAPD.
- ND: not determined.
"Locus name" column. RAPDs whose names end in "R": the polymorphic
band is amplified only from inbred strain C3 DNA. All others: the
polymorphic band is amplified only from inbred strain B DNA.
"Primers" column: RAPD primers from Operon Technologies, Inc.; the
"OP" prefix has been omitted and a "0" has been added before
single-digit primer number.
"Mg mM" column: Final Mg concentration in PCR reaction -- 2.5 mM -->
-- unless otherwise specified.
"Same as?" column: The two RAPDs share one PCR primer in common, give
polymorphic bands of the same size and map to the same location. They
may represent the same polymorphism, caused by the same DNA sequence
difference at the binding site for the primer shared in common.
"Other notes" column. psMiL: a pseudo-MIC limited RAPD, i.e., a
MAC-destined RAPD for which the PCR amplification signal from the MIC
is comparable is comparable in intensity to that from the MAC. This
makes it very difficult, if not impossible, to score MAC assortment
tests for these loci.
"Linkage group (lg)" column:
- Chromosome numbers are given according to the revised terminology; L
and R suffixes: left and right arms, respectively.
- UL: Unlinked to any locus. Assigned to its own linkage group, or to
another linkage group based on MAC coassortment.
- UM: Unmapped meiotically; tentatively assigned to linkage group
based on MAC coassortment.
"Coassortment group (cag)" column:
(+) or (-): RAPD that shows strong assortment bias to either the
band-positive or band-negative allele, respectively.
Key references to RAPD methods used:
- Application of the RAPD method to mapping the Tetrahymena
micronuclear genome:
Brickner J.H., Lynch T.J., Zeilinger D. & Orias E. (1996)
Identification, mapping and linkage analysis of randomly amplified
DNA polymorphisms in Tetrahymena thermophila. Genetics, 141:
1315-1325.
- Detailed mapping methods can be found in a chapter by Hamilton and
Orias, in the Asai and Forney in the Tetrahymena volume of Methods in
Cell Biology.
Loci: AS-GM RAPDs
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JB - JO RAPDs
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JP - LS RAPDs
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MJ - YD RAPDs
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Cbs polymorphisms
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Genes
Other pages:
Key to RAPD list
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Map location summary
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MIC/MAC Illustr. Guide
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Review of Tetrahymena Genetics
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Genomic Maps
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