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Catalog of Tetrahymena thermophila loci tested for linkage
The genetic mapping data presented here are courtesy of Eduardo Orias at The University of California at Santa Barbara. This information is also available on his Tetrahymena Genome Project Homepage.
(Updated by Orias 12-16-99)
This page contains links to technical specifications or phenotypes as
well as MIC & MAC map locations associated with each mapped locus.
Related resources in this web site:
Hierarchically-organized summary of MIC and MAC map locations
Illustrated guide to the relationship between genetic views of the
MIC and MAC genomes: linkage and coassortment groups.
Types of loci linked to on this page:
RAPD DNA polymorphisms. More than 350 loci are listed; they
constitute the framework of our genetic map. A key to the RAPD
polymorphisms and abbreviations used is included.
Chromosome breakage site polymorphisms (CBSP). These are B-C3
polymorphisms associated with particular chromosome breakage sites,
and they serve to anchor the physical map to the genetic map. The
cloning and identification of Cbs and associated polymorphisms around
the genome is actively in progress as a collaboration of the Bruns and
Orias labs.
Conventional genes. This list includes genes that happened to be
segregating in the panels used to map RAPDs or in ad hoc panels
constructed for special purposes to map other mutants. Current methods
for mapping mutant genes are described in a chapter by Hamilton and
Orias, in the Tetrahymena volume of Methods in
Cell Biology (Volume 62, 2000) by Asai and Forney. The linkage mapping of mutant genes will be expedited by
the development of universal tester panels (e.g., for deletion
mapping), recently started as a collaboration of the Bruns and Orias
labs.
Cloned-gene RFLPs. This list includes RFLPs mapped as a
demonstration project or for other special purposes. Note that the
eventual availability of the Tetrahymena genomic sequence will obviate
a genomic-scale effort to map cloned genes through associated RFLPs.
Loci: AS-GM RAPDs
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JB - JO RAPDs
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JP - LS RAPDs
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MJ - YD RAPDs
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Cbs polymorphisms
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Genes
Other pages:
Key to RAPD list
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Genetic Map Location Summary
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MIC/MAC Illustr. Guide
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Review of Tetrahymena Genetics
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Tetrahymena Genome Maps
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