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Illustrated Guide to Tetrahymena MIC & MAC Genetic Maps
The genetic mapping data presented here have been adapted from work by Eduardo Orias at The University of California at Santa Barbara. This information is also available on his Tetrahymena Genome Project Homepage.
The diagrams below illustrate the hierarchical relationship between
the MIC and MAC genomes and the genetic views of the two genomes: MIC
linkage groups, MAC coassortment groups, and various types of loci
mapped (genes, RAPDs and chromosome breakage site polymorphisms).
A. Hierarchical organization of MIC and MAC genetic maps associated
with a chromosome arm
Notes:
MIC Linkage groups. MIC chromosome arms are the physical basis of
linkage groups. Each arm will be eventually represented by a single
linkage group, as more loci are mapped.
MAC Coassortment groups. MAC chromosomes are the physical basis of
coassortment groups.
B. Detailed view of a segment of a linkage group
Additional Notes:
Loci names (but not types) are fictitious, but conform to
Tetrahymena Genetic Nomenclature.
Chromosome breakage sites (Cbs). These are the sites at which the
ends of MAC chromosomes are generated by site-specific fragmentation
of the MIC chromosomes during MAC differentiation. The Cbs is a
completely conserved DNA sequence of 15 base pairs, which is
necessary and sufficient for chromosome breakage. The two regions
flanking a Cbs are named as follows: the "A" region is downstream of
the 3' end of the Cbs strand that begins and ends with A, i.e., the
5' AAAGAGGTTGGTTTA 3' strand, while the "T" region is downstream of
the 3' end of the complementary Cbs strand, i.e., 5' TAAACCAACCTCTTT
3'. The "A" and "T" regions of a Cbs are specified only in MAC
maps. The identification and mapping of every Cbs in the MIC genome
and the ends of the two flanking MAC chromosome DNAs is in progress
and is the approach by which a physical map of the MIC genome is being
constructed.
Coassortment groups. (B) or (C) indicate strongly biased
assortment toward the B or C3 allele, respectively, observed in all
members of the coassortment group.
Genetic Loci. The Catalog contains a detailed description of
loci and phenotypes.
Loci: AS-GM RAPDs
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JB - JO RAPDs
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JP - LS RAPDs
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MJ - YD RAPDs
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Cbs polymorphisms
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Genes
Other pages:
Key to RAPD list
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Genetic Map Location Summary
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MIC/MAC Illustr. Guide
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Review of Tetrahymena Genetics
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Tetrahymena Genome Maps
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