Maximum Likelihood Micronuclear Chromosome Maps of
Tetrahymena thermophila
The genetic mapping data presented here are courtesy of Eduardo Orias at The University of California at Santa Barbara. This information is also available on his Tetrahymena Genome Project Homepage.
Contents:
- Explanation of the maps
- Overview - Distribution of linkage groups among chromosome arms
- Maximum likelihood maps
1. Explanation of the maps
- The loci mapped are described in the RAPD
and MIC Genetics Database.
- The basis for making MIC maps is explained in the Introduction to Tetrahymena Genetics.
(Key reference: Brickner et al., 1996.)
- Maximum likelihood orders and map distances (in cM) listed below
(and the corresponding map
coordinates) were obtained by using MAPMAKER. Distances were
corrected for undetected, predicted multiple crossovers
using the Haldane equation.
- Every member of a linkage group shows meiotic linkage at LOD >
3 with at least one other member.
- Only one representative locus is shown for most groups of loci
not yet resolved by recombination ("coincident
loci"). Other members of the group are listed in the RAPD and MIC Genetics Database,
and can be found by searching for the name of the representative
locus.
- Map orders are based generally on a panel of 32 meiotic
segregants; the orders are therefore soft over short
distances. A notable exception is the neighborhood of the mat locus in
2L, mapped with up to 200 meiotic segregants.
- More meiotic segregants (already available) can be typed by
anyone interested in a higher resolution map of
any particular region of the genome.
2. Overview - Distribution of linkage groups among chromosome
arms
1L CEN 2R 2L CEN 1R
--------------0-------------- --------------0--------------
1L-MJ10 2R-Mpr 2L-BB01R 1R-AT01
1L-SP13* 2R-JO66 2L-mat 1R-ChxA
3L CEN 3R 4L CEN 4R
--------------0-------------- --------------0--------------
3L-JB36 3R-AcpA 4L-AS01 4L-BR19 4R-LC01
3L-JO25 3R-JO06 4L-JB06
3L-MJ18R 3R-LC08 4L-QD02
3L-XS43 4L-QD02R
3L-EstB
5 X
----------------------------- ---------
5X-JB04 XX-KP04R
5X-JP02
5X-LC16a
L and R: Left and right chromosome arms. CEN: expected centromere.
Linkage groups cannot be assigned to chromosome 5 arms because the
required nullisomic is not available. Linkage
group XX has not yet been assigned a known chromosome for technical
reasons. The 1L.2R and 2L.1R chromosome arm
associations reflect the hypothesis of Bruns, Hanley-Cassidy &
Merriam (personal communication) regarding wild
type T. thermophila strains.
* Linkage group 1L-SP13 was originally assigned to chromosome 1L
but may be in 2R (currently under investigation).
3. Maximum likelihood maps
The maps extend vertically; click to reach quickly the headers for
the other chromosome arms (2R,
3R, 4L, 4R, 5, and unassigned X). The maps are drawn approximately
in scale. See the Map Coordinates page for
exact coordinates with which to draw your
own map. If you download the maps, they will look straight only when
using a font with equal spacing of characters,
such as Courier.
1L 1R 2L 3L
____________ __________________________ ____________ ____________
lg1L-MJ10 lg1R-ChxA lg1R-AT01 lg2L-mat lg3L-JB36
| | | | |
+1BR12 +1XS32 3.4+1JP18 3.4+1JO39R +1JB35
5.9| | +1JP18a +1YD20R |
+1MJ10 13.2| 7.2| 6.7| 16.8|
| | +1SP18R 0.0+1SN09 |
+1LC04 4.3| 0.0+1EO03R |
lg1L-SP13 | +1BR11 +1JP11 +1JB15
| 15.6| 10.7| 4.1| 7.4|
+1LR03 | | 2.0+1LS15 +1JB36R
9.6| | +1EM01 0.0+1JO16 |
| +1JB12 | 2.0+1JP33
+1SP13 6.1| | 0.0+1BD08R lg3L-EstB
| +EstA | 3.0+1AT03R |
7.4| 27.3| +1AT03 +EstB
+1BR20 | 4.2| |
| | +1JO09 |
2R 12.0| | 4.6| 21.1|
____________ | +1AT01 +1XS10 |
lg2R-Mpr +1LC09R | 5.3| |
| 7.8| 12.4| +1XS19 +1JP03
+1LC04R | | | |
| +1JO91R +1XS17 16.7|
| 5.1| 7.8| | lg3L-JO25
| 2.2+1JB30 | | |
26.9| 1.8+1JB14 +1JB05 +1JB11 +1JP23
| +Chx 7.7| 3.8| |
| | | 1.5+1AS02 18.4|
| 12.2| +1LR01 3.0+1KF02 |
+1CH02 | | 1.5+1EO01 |
11.1| +1KN02 14.2| 3.1+1PM08 +1JO25
| 5.9| | +mat |
+1KP02R +1ML03 +1JO03 4.6|
| 5.0| | 0.1+1KN03 lg3L-MJ18R
11.5| +1JO14 | +1XS24 |
| | 24.8| 5.1| +1MJ18R
+1JO71 14.3| | +1GM09 3.8|
| | | 3.9| +1JP23
11.5| +1LS07 | +1JO13R |
| 8.7| +1LC02 5.7|
+1YD13 | 9.0| +1CH01 lg3L-XS43
10.5| 2.1+1JB22 | 5.4| |
| +1JB08 +1JO05 +1XS23 +1XS43
+1AT04 6.7| 7.0| 5.4| 5.3|
| +1JP12a +1JO05R 0.0+1YD19 +1JB26
13.9| | 11.0| +1EM04 |
| | | 5.3|
+1LS21 20.5| +1JO56R 2.6+1JP34
| | 8.7| +1JB10 3R
| | | | ____________
19.6| +1JO18 +1SN03 14.2| lg3R-JO06
| | 8.7| | |
| 17.1| | +1JO06R +1LC09
+Mpr | 3.3+1LC10 9.0| |
| | +J083R | 12.8|
+1JO15 11.0| 0.1+1XS63R |
lg2R-JO66 | | 0.0+Pmr +1BD13
| 14.6| +1LC21 0.0+1EM10 |
+1XS27 | | +1SP09 17.9|
4.2| +1BD03 | 11.0| |
+1JO66 | 18.8| | |
| | | +1AT14 3.3+1BD28R
21.8| | | 3.2+1RK01R
| +1YD02 11.2| 3.3+1JO22
| 4.0| | +1JO06
+1LN02b +1JB28 +1BD06 7.2|
3.9| 4.6| 9.1| +1JO35R
+1LN01b +1JO65 | 8.7|
| | +1BD11 |
13.0| 8.2| +1LC21R
| | |
+1LS18R +1XS36
| 4.6| lg3R-LC08
+1RT01 |
3.9| +1GM03
+1JB03 |
| 12.1|
15.6| |
| +1LC08
| |
+1JP17 11.7|
| |
16.9| +1JB21
| |
|
+1SP19R lg3R-AcpA
| |
| +AcpA
23.7| |
| 16.8|
| |
| |
+1SP11R +1XS61R
| |
lg2L-BB01R
|
+1BB01R
|
15.5|
|
|
+1BR04
|
4L 4L-4R 4R 5
____________ ____________ ____________ __________________________
lg4L-AS01 lg4L-BR19 lg4R-LC01 lg5X-JB04 lg5X-LC16a
| | | | |
+1JB07 +1LS02 +1JO60 +1SN13 +1RK08
| | 6.9| | 8.4|
| 14.6| +1JP05 | |
20.6| | | 24.3| +1YD16
| +1SN11 | | 4.4|
| 6.7| | | +1BD34R
+1LS01 +1SN21 | | 9.3|
9.0| 4.4| 35.8| +1JB02 |
| +1KF04 | | +1LC16a
3.3+1JP06a | | 16.2| 8.8|
+1LS03 13.2| | | |
6.7| | | | +1GM08
3.2+1RK04 +1BR19 +1JO20R +1JB23 8.8|
3.2+1XS04 | | | |
3.2+1LS25 | 14.4| | +1JO68
3.2+1LS22 20.3| | 21.6| |
3.2+1MJ04 | +1JO31R | 16.8|
+1YD20 | 4.1| | |
| +1JO84R +1XS74R +1JP09 |
13.5| | | | +1SP16R
| 14.6| 15.6| 14.9| |
+1LS16 | | | 15.6|
4.4| +1KP05a | | |
+1AS01 5.2| +1LS26R +1BD25 |
7.1| +1MJ08 9.9| 10.8| +1LC02a
3.4+1JB18 5.0| | | |
3.4+1XS15 +1JB37 +1XS16 3.2+1JB17
+1XS04a 6.8| 6.9| 3.2+1XS11 lg5X-JP02
| +1AT11 +1JO37R +1LC18 |
15.0| 6.7| 10.6| 4.4| 3.6+1XS01R
| +1LK04 | +1YD07 +1JO75R
| 6.9| 3.3+1JO34R 9.2| |
+1JO27 +1RK03 3.3+1KP03R | |
4.9| | +1JB19 +1JP08d |
+1JO27a 6.8| 7.5| 32.8|
| +1LS29R | |
4.2| +1GM02 |
lg4L-QD02R +1LS30R 7.6| |
| | | |
+1QD02R 13.6| +1JB04 +1JP02
| | | 5.3|
12.6| +1LC26 15.5| +1JB29
| 7.8| | 5.4|
+1JB12R | | +1JP04
| +1LS14 +1JO10a 6.8|
| | 6.5| +1LS28R
18.9| 17.5| +1LC19 |
| | 10.4|
| | |
+1JO82R +1JO06a +1JP33a X
10.5| 5.1| 9.6| ____________
| +1JO62 | lgXX-KP04R
+1ML04R 5.0| +1AT06 |
| +1BD10 | +1JO04R
| 6.8| 14.7| 9.2|
23.5| +1AT04R | |
| 10.6| +1SP18aR +1KP04R
| | | 4.0|
| +1AT17 14.6| +1XS72R
+1AT18 | | |
| 15.1| +1JP01
| | 6.7|
24.5| | 3.2+1JO92R
| +1JO26 3.2+1SP18
| | 3.2+1JO01
| | 3.2+1JP12
+1KF03 18.6| +1EM02
| | |
| 14.7|
lg4L-JB06 +1JO36R |
| | +1JB33
+1JO02 | 7.7|
8.7| 24.9| |
| | +1XS49
3.3+1JB06 | 9.4|
3.2+1JP21 | |
+1ML02 +1LC06 +1XS53
| | |
15.3| 12.8| |
| | |
| +1LC20 26.7|
+1LC25 | |
| 11.8| |
13.2| | |
| +1BD32 +1AS02R
+1JO04 | 3.9|
| +1SP10
14.7| |
| 18.2|
+1LS09 |
| |
+1RK04a
lg4L-QD02 |
| 15.1|
+1BR21 |
4.0| |
+1JO52R +1BD34
| |
12.6| |
| 20.2|
+1QD02 |
| |
+1BR13
11.1|
|
+1JB24
7.0|
+1JO67
7.1|
+1JO56
11.0|
|
+1JB01
|
