Tetrahymena Stock Center
(Cornell University)

**Important Note: There is a new website for the Tetrahymena Stock Center at Cornell University. Please use the new forms available on their website for ordering and submitting Tetrahymena strains.

The National Tetrahymena Stock Center is located at Cornell University. The goal of the Stock Center is to provide a central point of distribution and offsite storage for the various Tetrahymena strains researchers have collected or generated. Funding for the Stock Center is provided by the National Institutes of Health (NIH) Grant 1 P40 RR019688-01A1.

Contact the Tetrahymena Stock Center: email: tetrahymenastockcenter@cornell.edu. Fax: (607) 253-3384 Phone: Dr. Ted Clark (607) 253-3042 Dr. Donna Cassidy-Hanley (607) 253-3407 Mailing Address: Tetrahymena Stock Center Microbiology and Immunology C5 152 Veterinary Medical Center Cornell University Ithaca, New York 14853

Commonly Used Strains Available From The Stock Center

Stock ID	Strain	Mic Genotype	Mac Genotype/Phenotype	Mating Type	Knockouts	Comments	Requires MTA1?	MTA Contact
MTP01	7 strains of different mating types		(variable)	I, II, III, IV, V, VI, VII		Panel of all 7 mating types. For use ONLY in mating type analyses. Included strains may vary.	No
SD00014	A*V		(V)	V		A strain star line. Lacks a genetically functional micronucleus.	No
SD00015	A*III (star)		(III)	III		A strain star line. Lacks a genetically functional micronucleus.	No
SD00021	B 2086		(II)	II		B strain wild type.	No
SD00022	B* (star)		(VI)	VI		B strain star line. Lacks a genetically functional micronucleus.	No
SD00023	B* (star)		(VII)	VII		B strain star line. Lacks a genetically functional micronucleus.	No
SD00104	CU357	chx1-1/chx1-1; mpr1-1/mpr1-1; Nulli 4	(CHX1; mpr1-1; cy-s, mp-r, IV)	IV		Not selected for 6mp-r. This is presumed genotype.	No
SD00174	CU427	chx1-1/chx1-1	(CHX1; cy-s, VI)	VI		Cycloheximide resistance functional heterokaryon.	No
SD00178	CU428.2	mpr1-1/mpr1-1	(MPR1; mp-s, VII)	VII		6-methylpurine resistance functional heterokaryon.	No
SD00189	CU438.1	pmr1-1/pmr1-1	(PMR1; pm-s, IV)	IV		Paromomycin resistance functional heterokaryon.	No
SD00231	CU482	Nulli 3; pmr1-1/pmr1-1	(CHX1; PMR1/pmr1-1; cy-s, pm-r, IV)	IV			No
SD00241	CU492	chx1-1/chx1-1; MPR1/MPR1; Nulli 5	(CHX1;mpr1-1; cy-s, mp-r, II)	II			No
SD00249	CU500	chx1-1/chx1-1; pmr1-1/pmr1-1	(CHX1; PMR1/pmr1-1; cy-s, pm-r, tr, II)	II			No
SD00271	CU522	mpr1-1/mpr1-1	(mpr1-1; mp-r, ory-r, IV)	IV	btu1-1::btu1-1M350K/ btu1-1::btu1-1M350K	Oryzalin resistant and taxol sensitivity are the result of the M350K mutation introduced into BTU1 by transformation.	Yes	Cornell
SD00481	CU725	chx1-1/chx1-1	(chx1-1; ory-r, cy-r, VII)	VII	btu1-1::btu1-1M350K/ btu1-1::btu1-1M350K	Oryzalin resistant and taxol sensitivity are the result of the M350K mutation introduced into BTU1 by transformation.	Yes	Cornell
SD00483	CU727		(ory-r, V)	V	btu1-1::btu1-1M350K/ btu1-1::btu1-1M350K	Oryzalin resistant and taxol sensitivity are the result of the M350K mutation introduced into BTU1 by transformation.	Yes	Cornell
SD00516	CU760	cdaA/cdaA; mpr1-1/mpr1-1; Nulli 1	(mpr1-1; mp-r, cdaA, IV)	IV			No
SD00532	CU776	chx1-1/chx1-1; Nulli 2	(CHX1; cy-s, VI)	VI			No
SD00625	IA134	cdaD2/cdaD2	(III)	III			No
SD00626	IA171	fatD1/fatD1	(III)	III			No
SD00632	IA264	gal1-1/gal1-1	(GAL1; gal-s)			2-deoxy-D-galactose resistance functional heterokaryon.	No
SD00634	IA277	con	(I)	I			No
SD00660	SB3539	chx1-1[C3]/chx1-1[C3]	(CHX1[C3]; cy-s, I)	I		C3 strain carrying cycloheximide resistance in micronucleus.	No
SD00663	SB3543	pmr1-1[C3]/pmr1-1[C3]	(PMR1[C3]; pm-s, I)	I		C3 strain carrying paromomycin resistance in micronucleus.	No
SD00665	SB3546	pmr1-1/pmr1-1	(PMR1; pm-s, VI)	VI		Paromomycin resistance functional heterokaryon.	No
SD00667	SB3549	chx1-1/chx1-1; pmr1-1/pmr1-1	(CHX1; PMR1; cy-s, pm-s, VI)	VI			No

1) Material Transfer Agreement (MTA): a contract that governs the transfer of tangible research materials between two organizations.